ABSTRACT
ObjectiveTo investigate the effect of Astragalin （AST） on apoptosis of cerebral cortex neurons in APP/PS1 transgenic mice. MethodsEighteen six-month-old male APP/PS1 transgenic mice were randomly divided into APP/PS1 group， APP/PS1+ 40 mg/kg AST group and APP/PS1+ 20 mg/kg Donepezil （DNP） group， with six mice in each group. At the same time， six male C57BL/6 mice were selected as the normal control group. After intraperitoneal injection of AST once a day and continuous administration for one month， we used Tunel staining to detect the apoptosis of neurons in the cerebral cortex of APP/PS1 mice； immunofluorescent staining to examine the expression of apoptosis-related proteins Bax， Bcl-2， Caspase9 and Cleaved-Caspase3 in the cerebral cortex neurons of APP/PS1 mice； Western blot method to evaluate the changes of the expression of Bax， Bcl-2， Caspase9 and Caspase3. ResultsTunel staining showed that 40 mg/kg AST and 20 mg/kg DNP both reduced the apoptosis of neurons in the cerebral cortex of APP/PS1 mice， AST with more significant inhibition effect. Immunofluorescent staining revealed that 40 mg/kg AST and 20 mg/kg DNP both inhibited the expression of Bax， Caspase9， and Cleaved-Caspase3， and icreased the expression of Bcl-2 in the cerebral cortex neurons of APP/PS1 mice. Western blot results further confirmed that 40 mg/kg AST and 20 mg/kg DNP both down-regulated the expression of Bax （P < 0.05， P < 0.05）， Caspase9 （P < 0.005， P < 0.05） and Caspase3 （P < 0.0001， P < 0.0001） ， and up-regulated the expresstion of Bcl-2 （P < 0.05， P < 0.05） in the cerebral cortex neurons of APP/PS1 mice. ConclusionsAST can inhibit the apoptosis of cerebral cortex neurons in APP/PS1 mice.
ABSTRACT
Objective:To compare the effects of different glucocorticoids on the pulmonary infection in patients with rheumatoid arthritis (RA).Methods: From January 2013 to February 2017,128 cases of patients with RA in our hospital were selected as the research object,all the cases were divided into observation group and control group with 64 cases in each group accorded to the random draw envelope principle.The observation group was given intra-articular injection of corticosteroid therapy,the control group was given oral glucocorticoid therapy,two groups were treated for 8 weeks.Results:The total effective rate of the observation group and the control group was 96.9% and 84.4% respectively,and the total effective rate of the observation group was significantly higher than that of the control group(P<0.05).The joint pain and joint swelling index of the observation group and the control group after treatment were sig-nificantly lower than that before the treatment (P<0.05),and the joint pain and joint swelling index of the observation group after treatment were significantly lower than that of the control group (P<0.05).The incidence of pulmonary infection in the observation group and the control group during treatment were 1.6% and 7.8%,respectively.The incidence of pulmonary infection in the observation group was significantly lower than that in the control group (P<0.05).The CRP and RF in the observation group after treatment were (10.11±3.19)mg/L and (50.22±19.82)U/ml that were significantly lower than the control group of (17.49±5.32) mg/L and (59.14±20.59)U/ml (P<0.05),while the CRP and RF values of the two groups after treatment was obviously lower than that before treatment (P<0.05).Conclusion: Compare with oral administration,intra articular injection of glucocorticoid for RA can reduce the incidence of pulmonary infection,inhibit the expression of inflammatory factors and RF,and promote the alleviation of clinical symptoms,thereby enhance the therapeutic effect.